Impact of long-term storage on stability of standard DNA for nucleic acid-based methods.
نویسندگان
چکیده
Real-time PCR is dependent upon a calibration function for quantification. While long-term storage of standards saves cost and time, solutions of DNA are prone to degradation. We present here the benchmark treatment for preservation of DNA standards, involving storage in 50% glycerol-double-distilled water, whereby a deviation of 0.2 threshold cycle (C(T)) values resulted after 100 days of storage.
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 48 11 شماره
صفحات -
تاریخ انتشار 2010